Detailed view for TGME49_235350

Basic information

TDR Targets ID: 262017
Toxoplasma gondii, cwfj family protein
Source Database / ID:  ToxoDB 

pI: 9.0804 | Length (AA): 821 | MW (Da): 90797 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04676   Protein similar to CwfJ C-terminus 2
PF04677   Protein similar to CwfJ C-terminus 1

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
490 600 1kpf () 16 116 14.00 0 0.44 0.29 -0.45
499 621 1ems (A) 305 433 20.00 0 0.78 0.38 -0.44
33 263 5cwp (A) 2 227 12.00 0.11 0.02 0.481164 -1.11
246 440 4ke2 (A) 3 192 10.00 0 0.03 0.328815 -0.63
257 351 1ol0 (A) 6 107 37.00 0.42 0.04 0.421613 -0.5
612 732 3p0t (A) 17 127 16.00 0.036 0.77 0.266181 0.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_129610)

Species Accession Gene Product
Arabidopsis thaliana AT1G56290   CwfJ-like protein
Babesia bovis BBOV_III005400   conserved hypothetical protein
Brugia malayi Bm1_44460   Cell cycle control protein cwf19
Caenorhabditis elegans CELE_B0361.2   Protein B0361.2, isoform B
Dictyostelium discoideum DDB_G0274367   cwfJ family protein
Drosophila melanogaster Dmel_CG9213   CG9213 gene product from transcript CG9213-RA
Echinococcus granulosus EgrG_000805400   CWF19 protein 2
Echinococcus multilocularis EmuJ_000805400   CWF19 protein 2
Homo sapiens ENSG00000152404   CWF19-like 2, cell cycle control (S. pombe)
Loa Loa (eye worm) LOAG_06477   cell cycle control protein cwf19
Mus musculus ENSMUSG00000025898   CWF19-like 2, cell cycle control (S. pombe)
Neospora caninum NCLIV_049800   hypothetical protein, conserved
Oryza sativa 4334057   Os03g0740700
Plasmodium berghei PBANKA_1435600   debranching enzyme-associated ribonuclease, putative
Plasmodium falciparum PF3D7_1220400   debranching enzyme-associated ribonuclease, putative
Plasmodium knowlesi PKNH_1439600   debranching enzyme-associated ribonuclease, putative
Plasmodium vivax PVX_123660   debranching enzyme-associated ribonuclease, putative
Plasmodium yoelii PY06625   hypothetical protein
Schistosoma japonicum Sjp_0000960   CWF19-like protein 2 homolog, putative
Schistosoma japonicum Sjp_0000970   expressed protein
Schistosoma mansoni Smp_042050   hypothetical protein
Toxoplasma gondii TGME49_235350   cwfj family protein
Theileria parva TP02_0406   hypothetical protein

Essentiality

TGME49_235350 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1435600 Plasmodium berghei Essential plasmo
TGME49_235350 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_235350 (Toxoplasma gondii), cwfj family protein
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